Tolerogenic phenotype of moDCs derived from patients with pancreatic cancer is associated with decreased PTPγ expression. (A) Allostimulatory function (MLR) of immature moDCs generated from patients with advanced or metastatic pancreatic carcinoma (n = 24, •) compared with control immature moDCs (n = 15, ○). Results are expressed as mean ± SD of [³H-TdR] uptake after 5 days of MLR culture in triplicate and show a reduced capability to stimulate T-cell proliferation by the former. CPM for T cells alone was 3963 ± 984. (B) IFN-γ production by allogeneic T cells stimulated by immature moDC from healthy donors (n = 15) and patients with advanced or metastatic pancreatic carcinoma (n = 15). Cytokine levels were detected by ELISA in cell-free supernatants collected from primary MLR before the addition of ³H-TdR. (C) PTPγ expression was increased in patients with metastatic pancreatic carcinoma (n = 9) compared with healthy donors (n = 11). (B-C) Median, 25th percentiles, 75th percentiles, minimum values, and maximum values are presented as vertical boxes and lines.