PDLIM2 prevents Tax-dependent NF-κB activation and HTLV-I viral transcription. (A-C) The indicated cells were transfected with Tax and κb driven luciferase reporter in the presence of increasing amounts of PDLIM2, followed by measure of luciferase activity. (D-F) The indicated cells were transfected with Tax and HTLV-I-LTR driven luciferase reporter in the presence of increasing amounts of PDLIM2, followed by measure of luciferase activity. (G,H) PDLIM2 wild-type (WT) or knockout (KO) MEFs were transfected with Tax and κb or HTLV-I-LTR driven luciferase reporter, followed by measure of luciferase activity. The protein expression levels of transfected Tax and endogenous Hsp90 were detected by direct IB. (I,J) PDLIM2 WT or KO MEFs were transfected with Tax and κb or HTLV-I-LTR driven luciferase reporter in the presence of increasing amounts of PDLIM2, followed by measure of luciferase activity. (K-L) 293 cells were transfected with Tax and κb or HTLV-I-LTR driven luciferase reporter in the presence of same amounts of PDLIM2 or its LIM deletion mutant, followed by measure of luciferase activity. The luciferase activities were presented as the percentile of that activated by Tax alone (denoted as 100) (A-F,I,J,K,L) or as fold induction relative to the basal level measured in WT MEFS (G,H). Error bars indicate standard deviations (n = 3).