Abnormal distribution of phenotypic HSCs and myeloid precursors in the bone marrow and spleen of diseased Ptpn11^D61Y mice. (A,B) Flow cytometric analysis of BM and splenic (A) LK subsets, including CMPs (LK-CD34⁺FcγRII/III^lo), GMPs (LK-CD34⁺FcγRII/III⁺), and MEPs (LK-CD34⁻FcγRII/III⁻) and (B) LSK subsets, LT-HSCs (LSK-Flk2⁻CD34⁻), ST-HSCs (LSK-Flk2⁻CD34⁺), and MPPs (LSK-Flk2⁺CD34⁺). Representative contour plots are shown. All values are the mean frequency of the parental gate. See Tables 1 and 2 for absolute numbers of each population. (C) LSK cells from BM or spleen of control and Ptpn11^D61Y mice were purified by FACS and stained with Hoechst 33342 (H), and Pyronin Y (PY). The mean percentages of cells in G₀ (H⁻PY⁻), sub-G₀G₁ (H⁻PY^lo), G₁ (H⁻PY⁺), and S/G₂M (H⁺PY⁺) are indicated. Representative contour plots are shown (n = 3; *P < .05).