Analysis of MM1R cells expressing GR mutants. (A) Western blot analysis of MM1R cells expressing 4 different GR mutants for GR expression with actin control. (B) Fold induction of GRE reporter expression with dexamethasone treatment of MM1R cells expressing wild-type GR or 4 GR mutants. The relative level of GRE reporter expression on dexamethasone treatment of MM1R-WT-GR cells was arbitrarily made 100%. Data from 3 experiments (mean ± SD). (C) Relative NF-κB transrepression with dexamethasone treatment. The data shown as NF-κB reporter inhibition relative to the repression observed in MM1R-WT-GR cells (arbitrarily 100%). Data are mean plus or minus SD of 3 separate experiments. (D) Western blot analysis of GR mutants, in the presence or absence of dexamethasone 10−6 M for 14 hours. (Top panel) Phospho-RAFTK. (Bottom panel) Actin.
