DNA ligase IIIα immunoprecipitates with WRN. Nuclear extracts from CML cell line K562 and EBV-transformed B-cell line NC10 were used to immunoprecipitate (A) DNA ligase IIIα, using WRN antibody, followed by Western blotting for DNA ligase IIIα (top panel) and WRN (bottom panel). (B) Reciprocal immunoprecipitation of WRN, using DNA ligase IIIα antibody, followed by Western blotting for WRN (top panel) and DNA ligase IIIα (bottom panel). Western blotting was also performed for negative control nonspecific antibodies.