NKp80 is expressed by certain HCMV-specific CD8 T cells. Shown are flow cytometric analyses of HCMV- and EBV-specific CD8 T cells for surface NKp80. Freshly isolated PBMCs from healthy HLA-A2⁺ donors were stained with NKp80-specific mAb 5D12 and HLA-A2/HCMV pp65_495-503 or HLA-A2/EBV LMP2_426-434 tetramers. CD8⁺ cells were gated for analysis. (A) Flow cytometric data of a selected donor with abundant NKp80 expression on HCMV-pp65–specific CD8 T cells (left) and a poor NKp80 expression on EBV-specific T cells (right). (B) Frequencies of NKp80⁺ cells among HCMV-specific and EBV-specific CD8 T cells. Among the 13 donors analyzed (age range, 31-59 years), 12 contained detectable HCMV-specific and 7 detectable EBV-specific T cells. Horizontal bars represent mean values.