miRNA profiles of APL primary blast upon ATRA treatment. Blast cells extracted from bone marrow of 3 different patients with APL were cultured for 6 days with ATRA (0.1 μM). (A) Viable cell counts during ATRA treatment were determined using Trypan blue. (B) Granulocytic differentiation was assessed by NBT dye reduction assay. (C-G) RT-qPCR analyses specific for RARB (C), miR-23a (D), miR-210 (E), miR-223 (F), miR-181a (G). Total RNAs were extracted each day of ATRA treatment for 4 days. Results are indicated as fold change, 1 being the value obtained in the absence of ATRA treatment (0 hour after treatment) for each patient (*P < .05).