SSL5 targets chemokine and anaphylatoxin receptors. (A,B) Binding of α-CXCR1 (●), α-CXCR2 (▾), and α-C5aR (*) mAbs to neutrophils, α-CXCR4 (♦) mAb to Jurkat cells, and α-CCR1 (■) and α-CCR2 (▴) mAbs to monocytes was measured to untreated (A) or neuraminidase-treated (B) cells after incubation with 0, 1, 3, or 10 μg/mL SSL5 for 15 minutes. (C) Binding of α-CXCR1 and α-CXCR2 mAbs to neutrophils and α-CCR1 and α-CCR2 mAbs to monocytes was measured after incubation with (□) or without (■) 10 μg/mL SSL11 for 15 minutes. Results are relative to buffer-treated cells and are expressed as mean plus or minus SEM of 3 independent experiments.