Figure 8
Figure 8. Inhibition of c-maf inhibits U266CS1 cell proliferation. (A) c-maf, cyclin D2, and cell surface integrin β7 expression was analyzed by immunoblotting and flow cytometry in U266CS1 cells 72 hours after transduction with a c-maf siRNA oligonucleotide. (B) U266CS1 cells transduced with c-maf siRNA (□) or control siRNA (■) were assayed by the incorporation of [H3]thymidine. A representative experiment performed in quadruplicate is shown. *P < .03. (C) Binding of U266CS1 transduced with c-maf siRNA or control siRNA to BMSCs was determined by an adhesion assay in a fluorescence plate reader. A representative experiment performed in triplicates was shown. a.u. indicates arbitrary units. *P < .03.

Inhibition of c-maf inhibits U266CS1 cell proliferation. (A) c-maf, cyclin D2, and cell surface integrin β7 expression was analyzed by immunoblotting and flow cytometry in U266CS1 cells 72 hours after transduction with a c-maf siRNA oligonucleotide. (B) U266CS1 cells transduced with c-maf siRNA (□) or control siRNA (■) were assayed by the incorporation of [H3]thymidine. A representative experiment performed in quadruplicate is shown. *P < .03. (C) Binding of U266CS1 transduced with c-maf siRNA or control siRNA to BMSCs was determined by an adhesion assay in a fluorescence plate reader. A representative experiment performed in triplicates was shown. a.u. indicates arbitrary units. *P < .03.

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