PP2A/C interacts with Bcl2 at its BH4 domain. Purified recombinant PP2A/C was incubated with purified WT, ΔBH1, ΔBH2, ΔBH3, or ΔBH4 Bcl2 deletion mutants in CHAPS lysis buffer at 4°C for 2 hours. The PP2A/C-associated Bcl2 was coimmunoprecipitated with an agarose-conjugated PP2A/C antibody and analyzed by Western blot using a Bcl2 antibody.