IL-17 production by FoxP3+/IL-17+ clones results in concomitant loss of suppressive function. The suppressive and effector functions of the clones were assessed in cocultures stimulated with αCD3/αCD2 beads or with 0.5 μg/mL αCD3 and APCs. On day 4, half the media in each well was removed, interrogated for content of IL-17 and IFNγ by ELISA, and replaced with 3H thymidine to monitor proliferation. Two representative clones for each pattern are shown. Data are representative of 2 independent cloning experiments. Panels show the ability of the clones to secrete IL-17 in response to the different stimuli (A), the levels of IL-17 induced in the respective clone cocultures (B), the ability of the clones to suppress Tresp proliferation (C), and the ability of the clones to suppress IFNγ production (D). **P < .01.
