Transfection of miR-17-19b-1 into B-cell lymphoma cell lines. (A) MiR-17, -19a, and -20a expression in Raji and SUDHL4 cells transfected with miR-17-19b-1 (Raji-m1 and SUDHL4-m1) or empty vector (Raji-c1 and SUDHL4-c1). Fold changes were determined by densitometry and are shown relative to the density obtained with cells transfected with empty vector, which was assigned a value of 1.00. (B) Cell proliferation assays carried out with 2 Raji and 2 SUDHL4 clones transfected with miR-17-19b-1 (Raji-m1 and Raji-m2/SUDHL4-m1 and SUDHL4-m2) or empty vector (Raji-c1 and Raji-c2/SUDHL4-c1 and SUDHL4-c2). Broken lines indicate Raji-c1 and Raji-c2. Cells were transfected using a lentiviral vector and plated at a density 10⁴ cells per well 5 to 7 days after EGFP selection. Cell proliferation was standardized to the fastest proliferating cells and was set to 100%. Symbols and bars indicate means and SDs of 2 independent experiments. (C) Cell-cycle analysis of Raji and SUDHL4 cells transfected with miR-17-19b-1 (Raji-m1 and SUDHL4-m1) or empty vector (Raji-c1 and SUDHL4-c1). Average (n = 3) percentage of empty vector–transfected Raji cells (c1) in G₁ phase: 29.3%, S phase: 61.3, and G₂ phase: 9.4%; and of miR-17-19b-1–transfected Raji cells (m1) in G₁ phase: 25.3%, S phase: 66.5%, and G₂ phase: 8.2%. Average (n = 3) percentage of empty vector–transfected SUDHL4 cells (c1) in G₁ phase: 74.7%, S phase: 20.6%, and G₂ phase: 4.7%; and of miR-17-19b-1–transfected SUDHL4 cells (m1) in G₁ phase: 55.8%, S phase: 39.9%, and G₂ phase: 4.3%. X-axis represents DNA content; y-axis, number of cells. Horizontal bars represent cell-cycle phases (G₁, S, G₂/M).