Validation of miRNA expression levels by qRT-PCR. The amount of miRNA-184, -206, -494, -363*, -155, 542-3p, and -9* in WM samples compared with normal controls, evaluated by qRT-PCR (□) and microbead miRNA profiling (■). Results are expressed as fold change of the miRNA expression in bone marrow WM CD19⁺ cells with respect to average miRNA expression from 3 healthy donors' bone marrow CD19⁺ cells (BM). qRT-PCR data were obtained using the ΔΔCt method, with normalization to the reference RNU6B microRNA. Bars represent SD.