Bzb increases TCF transcriptional activity in OBs and MSCs. C2C12 (A,F), C3H10T1/2 (B), MG63 (C), Saos-2 (D), and MSC from MM (E) were plated in 12-well plates and transiently cotransfected with 0.5 μg TOPflash and 50 ng pSV-β-galactosidase vector. The cells were treated with indicated concentrations of Bzb (A-E) or indicated concentration of MG132 (F) for 24 hours. The cell lysates were subjected to luciferase quantification as described in “Luciferase reporter assay.” Results represent the mean plus or minus SD of triplicate transfection of one representative experiment. * P < .05, ** P < .01, *** P < .001, **** P < .001 vs controls.