Transfer of ETCs that had been primed on recipient-derived DCs mediated a shift from mixed to full hematopoietic chimerism. No GVHD was observed. (A) GVHD was monitored on a clinical 10-point scale weekly for each animal. Each symbol represents the severest degree of GVHD that was observed in a single animal during the observation period. Groups were either treated with ETCs:R-DCs_pulsed (-♦-, n = 18-MC, 18-FC), ETCs:D-DCs_pulsed (-♦-,n = 9-MC and FC), ETCs:R-DCs_nonpulsed (-◇-,n = 9-MC and FC), naive DLI (-■-, n = 9 MC and FC), or PBS (-▴-, n = 9-MC, 18-FC). (B) MC were injected with 0.6 × 10⁶ C1498 cells intravenously on day 55 and 40 × 10⁶ effector cells on day 56. Chimerism was calculated by determining the percentage of CD45⁺H2D^d+ cells among the CD45⁺ cells. The values shown represent the mean chimerism of all mice per treatment group plus or minus SE.