Early adoptive transfer of ETCs triggers less severe GVHD in FCs than in MCs. ETCs:R-DCs_pulsed (-♦- n = 18-MC, n = 9-FC), ETCs:R-DCs_nonpulsed (-◇-n = 18-MC, n = 9-FC), ETCs:D-DCs_pulsed (-♦-, n = 18-MC, n = 9-FC), naive DLI (-■-, n = 18-MC, n = 9-FC), or PBS (-▴-, n = 18-MC, n = 9-FC) were given to either MCs (A,B) or FCs (C,D). Weight changes show more weight loss in MC animals that in FC animals. Mean weights plus or minus SE from each cohort are presented. GVHD was monitored weekly for each animal on a clinical 10-point scale. Each symbol represents the severest degree of GVHD that was observed in a single animal during the post transplant course. Mice treated with ETCs:R-DCs_pulsed developed less severe GVHD scores compared with all other treatment groups.