Induction of donor-specific transplantation tolerance by VAG-DCs. (A) SPCs from VAG-DC– and naive-DC–injected mice were isolated 100 days after transplantation, and from naive BALB/c mice (n = 3), and stimulated with irradiated donor-type (C57Bl/6) or third-party (C3H) CD90− APCs. Culture sups were collected after 96 hours and analyzed for the presence of IFN-γ using CBA assay (2-tailed t test: **P < .01). Error bars represent standard error between different mice (n = 3). (B) Balb/c recipient mice received a transplant of C57Bl/6 or C3H islets 1 day after injection of VAG-DCs (3 × 105) isolated from VAG539-treated islet graft–accepting mice. As control, nontreated Balb/c mice received a transplant of C57Bl/6 or C3H islets were used (2-tailed t test: *P < .05).
