Human VEGF-D expression in TG mice. Results of one representative real-time RT-PCR run with 3 mice were chosen to show the general pattern of TG expression in different tissues. F1-1 and F1-2 are mice of generation F₁; F3-1, -2 and -3 are TG mice of generation F₃. (A) All analyzed tissues are included in the same histogram. Because the scale is wide, the tissues that showed moderate to very low TG expression are shown with a lower scale in panel B. (C,D) Representative ELISA assays for hVEGF-D protein content in TG mice. (C) hVEGF-D protein in tissue lysates of F₁ and F₃ TG mice. (D) hVEGF-D protein in plasma of 5 F₁ mice. Error bars represent ± SD.