Blood and lymphatic capillary density in TG and control mice as shown by CD34, CD31, and LYVE-1 stainings. Images were taken from immunohistochemically stained 5-μm paraffin sections of mouse tissues and analyzed with Olympus Provis AX70 Microscope attached to Olympus ColorView 12 Camera. The software for imaging and analyses was AnalySIS (Soft Imaging System). (A) Representative figure from a TG mouse showed an increase in capillary density (CD). (B) In comparison, a normal capillary density was present in control mouse (CD34). (C) Representative figure from a TG mouse showed an increase in capillary density (CD31). (D) In comparison a normal capillary density was present in control mouse (CD31). (E) Lymphatic vessels were detected only in the interstitium accompanied by venules and arterioles in TG mice (LYVE-1). (F) Control muscles showed a similar pattern and number of lymphatic vessels (LYVE-1). Bars in panels A through F = 100 μm. (G) Capillaries/myocyte of nonoperated musculus rectus of the hVEGFD TG mice and the TG-negative controls. The mice were from generations F₁, F₂, and F₃. (H) Capillary density in myocardium of the hVEGF-D TG-positive and the TG-negative littermates. The mice were from generations F₁, F₂, and F₃. Error bars represent ± SD.