American Society of Hematology

Figure 1

$Figure 1. The B220−CD19− compartment contains lineage-restricted B-cell progenitors marked by transgenic expression of hCD25. (A) Representative FACS plots and purification scheme used to identify hCD25+ and hCD25− LIN−B220−CD19−CD127+FLT3+SCA1lowKITlow fractions. Numbers indicate percentage of gated cells. (B) The frequency (%) of cells composing colonies generated by coculture of single cells on OP9/OP9DL1 cells in the presence of indicated cytokines. The data are collected from 2 or more independent experiments. (C) FACS plots of the output from representative clones generated on OP9 (top) and OP9DL1 (bottom).$

The B220⁻CD19⁻ compartment contains lineage-restricted B-cell progenitors marked by transgenic expression of hCD25. (A) Representative FACS plots and purification scheme used to identify hCD25⁺ and hCD25⁻ LIN⁻B220⁻CD19⁻CD127⁺FLT3⁺SCA1^lowKIT^low fractions. Numbers indicate percentage of gated cells. (B) The frequency (%) of cells composing colonies generated by coculture of single cells on OP9/OP9DL1 cells in the presence of indicated cytokines. The data are collected from 2 or more independent experiments. (C) FACS plots of the output from representative clones generated on OP9 (top) and OP9DL1 (bottom).

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