Activation of the Igll1 promoter is associated with a reduced myeloid potential in progenitor cells. (A) The cloning frequency and the size of the colonies obtained after culture of single cells in a mix of myeloid cytokines. Error bars represent SEM. (B) Colonies detected (50× magnification) and the morphology of the cells generated during in vitro incubation with cytokines as visualized by May-Grünwald Giemsa staining (MGG; 500× magnification). Cells displayed as one picture are collected from the same slide, but the images of individual cells have been moved to fit the picture format. Images of tissue culture plate wells were taken with an Olympus IX70 microscope (with a LCPlanF1 20×/0.40 Ph1 objective; Olympus Optical, Tokyo, Japan) using a Nikon E4500 camera (Nikon Nordic AB, Solna, Sweden). Images of MGG-stained slides were acquired with an Olympus BX51 microscope (with a UPlanF1 100×/0.30 oil objective; Olympus Optical) using an Olympus DP70 camera and acquisition software (DP Controller 1.1.1.65; Olympus, Tokyo, Japan). (C) The number of PCR-positive samples generated after analysis of 16 randomly chosen clones by multiplex RT-PCR. Each horizontal line of dots indicates the gene expression pattern observed in a single investigated clone. Data presented are collected from 2 independent experiments.