Unconfirmed mutations at various positions throughout the V domain of subcloned sequences from subset 4 cases illustrating the “confirmed by another case” phenomenon. In the column headers, the number refers to the IMGT codon numbers, whereas the germline AA at each codon is indicated using the single-letter AA code. Within each column, the number of subcloned sequences containing a certain AA is indicated after the single-letter AA code. Unconfirmed mutations, which may be considered as “confirmed by another case,” are highlighted in bold. Dashes indicate that all subcloned sequences carried the germline AA. For example, the single K to R mutation at position IMGT/HFR3-90 in case P2920 is confirmed by 8 subcloned sequences in P3551 as well as K to R mutations in all subclones of cases P6520 and Swe181.