Embolization from WT, untreated and treated FVIII^null, and pFVIII/FVIII^null mice. (A) This panel from a venular WT injury illustrates how data were collected for these studies. Mice were injected with Alexa⁶⁴⁷-labeled anti-CD41 Fab fragments. A laser injury was made as indicated with a growing clot in red at that site, but data were collected at the indicated mask, where an embolus in red is passing by. The large arrow refers to the direction of blood flow. (B) Individual measurement of number of detected events for WT, untreated and treated FVIII^null, and pFVIII/FVIII^null mice are shown as well as the mean (red bar). Twenty separate injuries were analyzed for each study. Studies of arteriolar injuries are shown as filled symbols, while venular injuries are shown as empty symbols. Panels C and D are the same as panel B but show relative size of emboli and total amount of embolization, respectively.