Primary WM cells are characterized by low expression of PTEN and high expression of p-Akt and p-mTOR, compared with their normal cellular counterpart. (A) Immunoblotting for PTEN, p-Akt, p-mTOR, rictor, raptor, and β-actin expression in BM-derived CD19⁺ WM primary cells (WM), compared with normal primary CD19⁺ cells (Norm). (B) Purified cRNA (15 μg) isolated from primary BM-derived CD19⁺ WM primary cells (WM) and normal primary CD19⁺ cells (Norm) was hybridized to HG-U133Plus2.0 GeneChip (Affimetrix). Fold change is shown by the intensity of induction (red) or suppression (blue).