Expansion of human CD1d-LPC⁺ T cells in culture. (A) Expansion of CD1d-LPC⁺ cells in serum-containing media: representative FACS plot showing the presence of LPC specific T cells in freshly isolated PBMCs or after coculture with DC pulsed with or without LPC in culture media with 5% pooled human serum. Numbers in the quadrant correspond to percentage cells of total CD3⁺ cells, and data shown are gated for live cells. (B) Summary of experiments (n = 8) with unpulsed-DC or LPC-pulsed DC in serum-containing culture media. Each dot corresponds to percentage of CD1d-LPC⁺ cells per total CD3⁺ cells, and each line corresponds to data before and after 2-week expansion. (C) Expansion of CD1d-LPC⁺ cells in serum-free media: representative FACS plot showing the presence of LPC specific T cells in freshly isolated PBMCs or after coculture with DC pulsed with or without LPC in serum free media. Numbers in the quadrant correspond to percentage of cells of total CD3⁺ cells, and data shown are gated for live cells. (D) Summary of experiments (n = 4) with unpulsed DC or LPC pulsed DCs in serum-free media. Each dot corresponds to percentage of CD1d-LPC⁺ cells per total CD3⁺ cells, and each line corresponds to data before and after 1-week expansion.