Engraftment of donor-derived cells in the liver. (A) A total of 250 000 Lin⁻ cells were transplanted in HA mice after 1 day of the administration of normal saline (control) and acetaminophen. The presence of eGFP⁺ cells was determined by flow-cytometric analysis. Representative dot-plots show an increase in eGFP⁺ cells at different times after transplantation in acetaminophen-injected mice. (B) IHC analysis of liver sections of saline (left) and acetaminophen (right) injected mice after 5 months of transplantation. A representative photomicrograph shows the presence of eGFP⁺ cells near the central vein of acetaminophen-injected mice (original magnification ×20). (Inset) Magnified region of the section. (C) The percentage increase of eGFP⁺ cells in the whole liver at different times of transplantation. n = 4 for each time point. *P < .05.