Quantitative analysis of GFP⁺albumin⁺ and GFP⁺vWF⁺ cells at different times of transplantation. A single-cell suspension of liver was analyzed by flow cytometry. (A) Representative dot-plots for GFP⁺albumin⁺ and GFP⁺vWF⁺ cells at different times: (top panel) R1, R2, and R3 regions for eGFP⁺, albumin⁺, and GFP⁺albumin⁺ cells, respectively; (bottom panel) R1, R2, and R3 regions for eGFP⁺, vWF⁺, and GFP⁺vWF⁺ cells, respectively. (B) The percentage of eGFP⁺ cells that express albumin at different times. The results show that more than 85% of donor-derived cells expressed albumin (n = 4, for each time point). (C) The percentage of eGFP⁺ cells that express vWF at different times. The results show that 4% to 8% of donor-derived cells expressed vWF (n = 4 for each time point). (D) The liver and renal function test in HAT mice. Mice at different times of transplantation were evaluated in terms of liver and renal functions. Serum ALT, plasma creatinine, and BUN levels of HAT mice were compared with those levels in WT mice.