Serum Flt3-L levels are elevated after DC depletion. (A-B) Serum Flt3-L concentration in mice with a normal or depleted DC compartment. Sera of mice was collected at the indicated time points of DT treatment and analyzed for Flt3-L by enzyme-linked immunoabsorbent assay. (A) Single DT injection at day 0 (); (B) daily injections of DT for a period of 10 days. The y-scale on the left indicates the concentration of Flt3-L in serum (black and white circles); the y-scale on the right indicates the percentage of DC in CD11c.DOG mice (grey circles). The number of DCs without DT treatment was set at 100%. Shown is a representative of 3 experiments (n = 4 mice per group).