Induction of EBNA1-specific T-cell responses by αDEC-205-EBNA1 targeting to DCs. (A) PBMCs from blood donors were stimulated with mature autologous monocyte-derived DCs that were pulsed with medium, EBNA1 peptides, 1 μg/mL αDEC-205-EBNA1, or control Ig-EBNA1 using poly (I:C) as an adjuvant. After 9 days, frequencies of IFN-γ⁺ CD3⁺ T cells were determined by intracellular staining after EBNA1 peptide restimulation (top row). After a second round of stimulation with an EBNA1 peptide library on day 9, proliferation was evaluated by CFSE dilution on day 15 (bottom row). Representative data of 17 experiments are shown. Percentages on plots are of total cells within respective gates. Summary of the EBNA1-specific T-cell responses for 17 donors evaluated (B) by IFN-γ⁺ secretion on day 9 and (C) by CFSE dilution on day 15. Horizontal lines represent medians.