Expression of DAF, Crry, and CD59 on murine platelets. FACS analysis of DAF (A) and Crry (B) on wild-type mouse platelets. Platelets from DAF^−/−/C3^−/− and Crry^−/−/C3^−/− mice were used as negative controls. (C) FACS analysis of CD59a levels on WT (614 ± 32 molecules/cell) and various knockout mouse platelets. Platelets from DAF^−/−/CD59a^−/− mice were used as negative controls. (D) Comparison of the number of DAF and Crry molecules on WT, DAF^−/−/C3^−/−, and Crry^−/−/C3^−/− mouse platelets. WT and Crry^−/−/C3^−/− mouse platelets expressed 286 (± 98) and 282 (± 57) DAF molecules/platelet, respectively. WT and DAF^−/−/C3^−/− mouse platelets expressed 294 (± 86) and 274 (± 71) Crry molecules/platelet, respectively. Error bars represent SEM.