Higher percentages of Vδ1⁺ γδ T cells in CMV⁺ vs CMV⁻ healthy subjects. The percentage of γδ T cells in the freshly collected peripheral blood of 80 CMV⁻ and 106 CMV⁺ blood donors was determined by flow cytometry. Representative raw data are presented in Figure S1. (A) The medians, 25th and 75th percentiles (bottom and top of histograms), and 10th and 90th percentiles (bottom and top bars) of γδ, Vδ2⁺, Vδ1⁺, Vδ3⁺ T cells in the T-lymphocyte population, and CD8highCD57⁺ cells in the αβ T-cell population. (B) In the course of the study, blind phenotyping of Vδ1⁺ and Vδ3⁺ γδ T-cell populations was performed twice for 5 subjects with an interval of several weeks. ○ represent seronegative subjects; , seropositive subjects; □ and , subject presenting a CMV seroconversion between first and second sample.