The expanded Vδ2⁻ γδ T cells in CMV-experienced donors are mainly TEMRA cells. Freshly collected blood from CMV⁻ or CMV⁺ blood donors was stained for 6-color flow cytometric analysis with mAbs directed against CD3, γδ TCR, Vδ2 and activation markers or NKR. Raw data are presented in Figure S3. (A) Percentages of Vδ2⁺ or Vδ2⁻ γδ T cells positive for CD27 and/or CD45RA in 9 CMV⁻ () and 9 CMV⁺ donors (●). The short line represents the mean percentage for each cohort. (B) Mean (± SD) percentages of positive cells (for CD57, CD158a/h, b/j, i, e, CD94, NKG2A, NKG2C, or NKp80) or negative cells (for CD27) among Vδ2⁻ γδ T cells from CMV⁻ or CMV⁺ blood donors.