CD56⁺ cells from human peripheral blood harbor CD14⁺ cells, which resemble intermediate-sized lymphocytes. CD56⁺ cells were isolated from PBMCs using CD56 microbeads and magnetic-activated cell sorting technology. Purified CD56⁺ cells were stained for CD56 or CD14 and analyzed with a flow cytometer. (A-B) Step-wise arbitrary gating of subsets with increasing FSC identified a CD14⁺ cell population with scatter properties characteristic of intermediate-sized lymphocytes. (C) Plotting of CD14 expression against FSC resulted in the segregation of the CD14⁺ population from the lymphocytes. (A,C) Backgating of CD14⁺ cells (C) in the multicolor mode visualized the CD14⁺ population in the FSC/SSC histogram within the lymphocyte population (A gray dots). Results shown are from 1 representative donor (n = 21).