DFO-induced ferritin loss is prevented by chloroquine treatment. HEK293T-Fpn cells were incubated with FAC (10μM Fe) for 24 hours followed by incubation in the absence or presence of 10μM ponasterone A, 100μM DFO, desferasirox, or deferriprone. Chloroquine was added at the final concentration of 100μM. After 10 hours, cells were harvested. The ferritin content was determined by ELISA. Error bars represent SD from 3 different experiments.