DFO does not induce pH changes in the lysosome. HeLa cells were incubated with fluorescein dextran for 6 hours and then incubated in the absence of dextran for 2 hours. Cells were then incubated with or without chloroquine, DFO, DFO saturated with iron (DFO-Fe), or DFO-dextran. Images were acquired on an epifluorescence microscope. The data are representative images from each condition found in 3 separate experiments.