Inhibition of DFO-induced autophagy results in ferritin degradation in the proteasome. HEK293T-Fpn cells were incubated with FAC (10μM) for 24 hours followed by incubation in the presence or absence of 10μM ponasterone A (PoA) for 12 hours. Cells were incubated with or without 3-methyladenine for 8 hours in the presence or absence of 100μM chloroquine or 10μM MG132. FAC-loaded cells were also incubated with DFO, desferasirox, or deferriprone at the final concentration 100μM with or without 3-methyladenine for 8 hours in the presence or absence of 100μM chloroquine or 10μM MG132. Cells were harvested and ferritin content was determined by ELISA. Error bars represent SD from 4 different experiments.