Ferritin degradation is mTOR and LAMP 1-2 independent. (A) Wt and LAMP1^−/−2^−/−cells were incubated with or without FAC (10μM) for 24 hours. Cells were then incubated with 100μM DFO or desferasirox in the presence or absence of 100μM chloroquine for 8 hours. Cells were harvested and ferritin content was determined by ELISA. Error bars represent SD from 3 different experiments. (B) HeLa cells treated as in panel A were incubated in the presence or absence of 100nM rapamycin for 12 hours. Cells were harvested and ferritin content was determined by ELISA. (C) Cells as in panel B were stained for LC3B and the percentage of LC3B-positive cells was determined. Error bars represent SD from 3 different experiments.