Analysis of alternative splicing. (A) Splicing in normal persons and CEP patients. Genomic positions of the alternative splice sites are indicated as base pairs before the first residue of exon 10 and the positions of the lariat “A” in their respective putative BPSs are indicated as base pairs before the first exonic residue of the nearest exon. The sizes of the cryptic exon inserts noted in Figure 2 are listed in the right column. (B) Design of TaqMan assays for quantitation of the normal and alternatively spliced transcripts. The primer sequences are listed in supplemental Table 2.