TG: calibration of the anticoagulant effect. (A) TG as a function of the indicated concentrations of 23H9 and 28F4 added to NPP, initiated by 5pM tissue factor (TF) and 1μM phospholipids (PLs). (B) Calibration curve with 7 calibration plasmas (NPP spiked with decreasing concentrations of 23H9 and 28F4) constructed for the normalized PH/LT ratio as a function of antibody concentration, defined as LAC arbitrary units. One LAC AU/mL was defined as the PH/LT of the calibration plasma spiked with the combination of 23H9 (100 μg/mL) + 28F4 (40 μg/mL). The normalized PH/LT measured in the native NPP (1 on y-axis) has no LAC activity. (C) Normalized PH/LT ratio measured for NPP spiked with 23H9 anti-β2GPI IgG (100 μg/mL) and 28F4 (40 μg/mL; Mab in NPP), and for 2 representative patient plasma samples, as a function of the indicated dilution factor.