Hematopoietic activity in intra-BM islet-infused mice after infection with LCMV. (A) Mice receiving islets intra-BM were infected at approximately 1 year of age with 2 × 10⁵ plaque-forming units of LCMV. Glycemia, platelet, and WBC counts were measured either daily or every other day. (B) Femurs collected from intra-BM islet-transplanted LCMV-treated mice and from controls (age-matched C57BL/6 mice either infected with LCMV or not) were harvested on day 8 postinfection and monitored for the relative content of BM precursors and the presence of insulin-producing β cells. (C) Viral titers in blood harvested at the indicated time points after infection were measured by a reverse transcription–polymerase chain reaction–based assay with a detection limit of 10³ copies of LCMV RNA (genome equivalents [GE]) per mL of total blood. Results are expressed as means of triplicate measurements ± SD. Assessment by intracellular IFN-γ staining of the percentage of glycoprotein 33-specific CD8⁺ T cells recovered from spleens harvested at day 8 postinfection. Results are expressed as mean ± SD.