Characterization of HSCs and progenitors in KRNxG7 spleen. (A) Spleen from 1-, 2-, and 6-week-old B6xG7, KRN, and KRNxG7 mice were subjected to FACS analyses for cKit, Sca1, and lineage markers. The frequency ± SD of CD45⁺KSL⁻ is shown on the y-axis (n ≥ 5). (B) BM and spleen cells from 6-week-old KRN and KRNxG7 mice were subjected to hematopoietic replating assay (n = 3). Hematopoietic colonies were counted 7 to 10 days after replating. (C) Lin⁻ spleen cells from B6xG7 (CD45.2xCD45.2) or KRNxG7 (CD45.2xCD45.2) were transplanted into lethally irradiated B6xG7 (CD45.1xCD45.2) mice. Survival rate of the recipients is shown on the left. CD45.2⁺ cells were analyzed 3 months after transplantation. Representative FACS data are shown on the right. ***P < .001.