T cells maintain antilymphoma activity after in vivo pretreatment of donor mice with a CD28-SA. (A) Survival of lethally irradiated BALB/c mice inoculated with BCL1 lymphoma cells 4 hours before TCD BM cell transfer. 106 T cells (A) or 105 T cells (B) from donor mice treated as indicated were transferred together in 1 experiment with the TCD BM cells and in the other 4 days later. Both experiments rendered similar results. Therefore, data from the 2 experiments were pooled (n = 12). Differences between mice receiving donor T cells and the TCD BM only group were subjected to statistical testing. Numbers in parentheses indicate animals with end-stage lymphoma/animals analyzed. (C) The histograms show the detection of BCL1 idiotype-positive cells (black) in the spleens of mice from the indicated groups. A control staining without the anti-idiotypic antibody is depicted in gray.
