Thrombin generation in PPP and PRP from patients with severe congenital FV deficiency. PPP and PRP from patients PD I, PD II, PD III, and PD VII were triggered with 1 (light gray), 5 (middle gray), 10 (dark gray), or 50 (black) pM of TF, and thrombin generation was determined as described in “Thrombin generation assays.” Thrombin generation in PRP was measured without the addition of any platelet agonist and after preactivation of platelets with collagen (10 μg/mL) or Ca²⁺-ionophore (20μM). A representative control is shown for comparison.