Figure 3
FV dependence of thrombin generation in PRP from a FV-deficient patient. (A) Effect of an anti-FV antibody on thrombin generation in FV-deficient PRP. PRP from patient PD III was triggered with 50pM of TF in the presence of Ca2+-ionophore and thrombin generation was measured in the absence of an anti-FV antibody (●) and in the presence of 8 μg/mL (○), 28 μg/mL (▴), 60 μg/mL (▵), and 108 μg/mL (◇) of anti-FV antibody. (B) Effect of APC on thrombin generation in FV-deficient PRP. PRP from patient PD III was triggered with 5pM of TF in the presence of calcium ionophore and thrombin generation was measured in the absence of APC (●) and in the presence of 50nM of APC (▵).
