Genetic characterization of a patient with AML-M5 and a t(12;18)(p13;q12) involving ETV6. (A) FISH analysis indicating the breakpoint on 18q12: probe RP11-252G8 (green) splits and hybridizes in both der(18) and der(12). (B) Analysis by quantitative RT-PCR of the SETBP1 expression in samples of the patient at diagnosis, posttreatment control, and relapse. (C) RACE results showing the 2 different fusion transcripts detected. Sequences from ETV6 are in italics; the putative exons from chromosome 18 are underlined.