Ectopic expression of SETBP1 induces increased full-length SET levels and inhibits PP2Ac. (A) Western blot showing endogenous SET expression in HEK293 cells transfected with SETBP1-GFP, an empty vector as a control, or nontransfected cells. (B) Analysis by Western blot showing SET in HEK293 cells transfected with different regions of SETBP1. SHR indicates SKI homologous region; and SETBD, SET binding domain. (C) Western blot and densitometry analysis showing the effect of ectopic SETBP1 over PP2A expression in HEK293 cells. (D) Western blot and densitometry analysis showing the effect of ectopic SETBP1 over PP2A phosphorylation levels of the tyrosine-307. (E) PP2A phosphatase assay in HEK293 cells transfected or not with SETBP1.