Figure 1
CFH and FHL-1 content in normal platelets, platelets lacking α-granules, and human megakaryocytes. Immunoblot of platelet and megakaryocyte whole-cell lysates consisting of washed platelets (107 platelets/lane) from a normal person (Normal platelet), an ARC syndrome patient (ARC platelet), and serum-free cultured megakaryocytes (Normal MK). The blot was initially probed with a rabbit polyclonal antibody capable of detecting CFH and FHL-1 and subsequently with antibodies specific for TSP1 and β-actin, used as α-granule content and protein-loading indicators, respectively. Both CFH and FHL-1 are present in normal megakaryocytes and platelets, and also in ARC platelets lacking α-granules (near absence of the α-granule protein TSP1).
