T cells from allogeneic CD8α⁺ DC–vaccinated mice. Reduced expansion and proinflammatory cytokine secretion were shown in vitro in BALB/c mice that were immunized with diluent control or allogeneic B6 BM-derived CD8α⁺ DCs or CD8α⁻ DCs as described in “Methods.” BALB/c CD90⁺ T cells (2 × 10⁵/well) were then harvested from them and cultured for 96 hours with either syngeneic or irradiated (3000 cGy) allogeneic B6 BM DCs (1 × 10⁴/well). During the final 16 hours of a 96-hour culture, cells were pulsed with [³H] thymidine and assayed for (A) proliferation. Supernatants were collected at 80 hours and assayed by for (B) IL-2, (C) IFN-γ, and (D) IL-10. Error bars represent SE. *P < .05 between diluent control and allogeneic CD8α⁺ DC–vaccinated groups. Data are from one of 3 similar experiments. *P < .05 between diluent control and allogeneic CD8α⁺ DC–vaccinated groups.