Immunization with allogeneic CD8α⁺ DCs reduces T-cell–proliferative response without altering their rate of apoptosis. T cells were harvested from BALB/c mice that were immunized with diluent or B6 BM-derived CD8α⁺ DC and CD8α⁻ DC subsets as described in “Methods.” BALB/c CD90⁺ T cells (2 × 10⁶/well) were then stained with CFSE and stimulated in vitro with B6 BM DCs for 96 hours and analyzed by flow cytometry. (A) Gated for fluorescein isothiocynate–conjugated CFSE and allophycocyanin-conjugated CD3⁺ T cells. Data are representative of 1 of 4 independent similar experiments. Combined data for the nonimmunized, CD8α⁺ DC–vaccinated, and CD8α⁻ DC–vaccinated are 0.77% ± 0.15%, 24.1% ± 2.4%, 4.7% ± 1.6%, and 18.1% ± 0.6%, respectively (P < .04, nonimmunized vs CD8α⁺ DC). (B) PE-conjugated annexin V and allophycocyanin-conjugated anti-CD3. P = not significant between the groups. Data are representative of 1 of 3 independent similar experiments.