The SVF contains phenotypic and functional HSPCs. (A) Representative flow cytometric profiles of LSK cells in the SVF, BM, and peripheral blood (PB). One million SVF, BM, and PB cells were pregated for live cells, and then Lin⁻ cells were analyzed for Sca-1 and c-Kit expression. The percentage of LSK in the total SVF (n = 5) BM (n = 5) or PB (n = 5) is expressed as mean ± SD. (B-E) Freshly isolated SVF (n = 5) or BM (n = 5) was plated on methylcellulose-based medium and incubated for 14 days for CFC assay. (B) The frequency of colony-forming cells is shown. The bars represent mean ± SD (n = 5); *P < .05 vs BM. (C) Types of colonies are photographed. Scale bars, 100 μm. (D) The colonies are classified by morphologic criteria and calculated in percentages. (E) Flow cytometric analysis for CD45 expression in cells from SVF-derived colonies.